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. 2021 Mar 4;70(3):311–321. doi: 10.1538/expanim.20-0160

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©2021 Japanese Association for Laboratory Animal Science

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)

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Fig. 4. — (NADPH) oxidase homolog 1 (NOH-1) increased oxygen and glucose deprivation/reperfusion (OGD/R)-induced cell apoptosis. To evaluate the effect of NOH-1 on cell apoptosis, relevant functional assays were performed. (A) Cell viability was detected using an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. (B) Cell apoptosis was determined by Terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL), and the apoptosis rate was analyzed. (C) Western blot was performed to detect the expression of Bax, Bcl2, pro-caspase-3, cleaved caspase-3, pro-poly(ADP-ribose) polymerase (PARP) and cleaved PARP. All results are presented as means ± SD, and they were analyzed by one-way ANOVA. ^XXXXP<0.0001, compared with the Control. ^VVP<0.01, compared with OGD/R + negative control (NC) mimics. ^VVVP<0.001, compared with OGD/R + NC mimics. ^VVVVP<0.0001, compared with OGD/R + NC mimics. ^NP<0.05, compared with OGD/R + miR-145-5p mimics + empty vector. ^NNP<0.01, compared with OGD/R + miR-145-5p mimics + empty vector.