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Defective activation of SAPK in TPA-resistant myeloid leukemia cells. (A) TUR and HL-525 cells were treated with 16 nM TPA for the indicated times or with 40 ng of okadaic acid (OA) per ml for 6 h. (B and C) U-937 and TUR cells (B) or HL-60 and HL-525 cells (C) were treated with the indicated concentrations of OA for 6 h. Anti-SAPK antibody immunoprecipitates were assayed for phosphorylation of GST-Jun.
