TABLE 1.
Effects of TPA on differentiation of TUR and HL-525 cell transfectantsa
| Cell type | Cell growth (% of control) | Adherent cells (% of total cells) | % of cells showing NSE staining |
|---|---|---|---|
| U-937 | 30 | 96 | >90 |
| TUR/neo-1 | 87 | 2 | <5 |
| TUR/neo-2 | 83 | 5 | <5 |
| TUR/PKCβII-1 | 49 | 93 | >75 |
| TUR/PKCβII-2 | 47 | 91 | >75 |
| HL-60 | 29 | 97 | >90 |
| HL-525/neo-1 | 101 | 3 | <5 |
| HL-525/neo-2 | 97 | 3 | <5 |
| HL-525/PKCβII-1 | 35 | 89 | >85 |
| HL-525/PKCβII-2 | 41 | 91 | >80 |
a
Cells were seeded onto six-well tissue culture dishes (2 × 105 cells/well) and cultured in the presence or absence of 16 nM TPA for 48 h. Viability, as determined by trypan blue exclusion, was >95% for adherent and nonadherent cells. NSE staining was performed at 72 h. Results are expressed as the mean of three determinations (standard error, <10%).