Fig. 6.

Topical treatment of A. cruentus leaves with siRNAs targeting CHL-H showed that 22-mers produced a stronger phenotype compared to 21-mers and resulted in more transitive sRNAs. siRNAs were applied to 14-day-old plants (6 reps), and treated leaves were abraded with dry particles. a treated leaves were photographed 7 days after treatment, and leaves with representative phenotypes for the treatment are shown. b Leaf silenced area was quantified using Image J, and plotted with standard errors. Letters under the bars indicate significant difference by Student’s t test, α < 0.05. c small RNAs (19–25 nt) were mapped to the targeted CHL-H transcript. The red bars indicate sense and antisense reads mapping to site corresponding to the applied siRNA. Blue bars represent sense and antisense small RNA reads mapped 3′ to the applied siRNA. CP20M, counts per 20 million