Table 1.

siRNAs targeting GFP or control sequences

Duplexes	Sense (passenger) strand (5′–3′):antisense (guide) strand (5′–3′)	Figure/Table
Controls
A. palmeri EPSPS	AUGCCAGAUGUUGCUAUGACUCUU:AAGAGUCAUAGCAACAUCUGGCAU	Figures 1, 2, 3
Scrambled	GCCGAUCAACCUCAAACUUAAU:GAAGUUUGAGGUUGAUCGGCUU	Figure 6
N. benthamiana 16C-GFP
GFP_21/22	GGC-UCAAAGCCAACUUCAAAA:UUGAAGUUGGCUUUGAUGCCGU	Figures 1, 2, 3, 4, 5; Table 3
GFP_20/20	CAUCAAAGCCAACUUCAAAA:UUGAAGUUGGCUUUGAUGCC	Figure 2
GFP_21/21	GCAUCAAAGCCAACUUCAAAA:UUGAAGUUGGCUUUGAUGCCG	Figures 2, 4; Table 3
GFP_22/22	GGCAUCAAAGCCAACUUCAAAA:UUGAAGUUGGCUUUGAUGCCGU	Figures 2, 3, 4; Table 3
GFP_22/21	GCACUCAAAGCCAACUUCAAAA:UUGAAGUUGGCUUUGA-UGCCG	Figures 2, 4; Table 3
GFP_21/22_mut 9–11	GGC-UCAAACGGAACUUCAAAA:UUGAAGUUCCGUUUGAUGCCGU	Figure 3
GFP_21/22_mut 10–12	GGC-UCAAUCGCAACUUCAAAA:UUGAAGUUGCGAUUGAUGCCGU	Figure 3
GFP_21/21_v2	GCAUCAAAGCCAACUUCAAGA:UUGAAGUUGGCUUUGAUGCCG	Figure 5

Letters in bold indicate introduced mutations