Table 2.

siRNAs targeting the N. benthamiana CHL-I gene

Duplexes	Sense (passenger) strand (5′–3′):antisense (guide) strand (5′–3′)	Figure/Table
Magnesium cheletase I
CHL-I_21/22	GGG-CCGUGAGAGAUGCAGAAA:UCUGCAUCUCUCACGGUCCCCA	Figure 3
CHL-I_21/22_mut 9–11	GGG-CCGUGGAUGAUGCAGAAA:UCUGCAUCAUCCACGGUCCCCA	Figure 3
CHL-I_21/22_mut 10–12	GGG-CCGUCGAAGAUGCAGAAA:UCUGCAUCUUCGACGGUCCCCA	Figure 3
CHL-I_21/22_miRNA-like	GGG-CCGUGUGAGUUGCAGUGA:UCUGCAUCUCUCACGGUCCCCA	Figures 3, 4; Table 3
CHL-I_22/22_miRNA-like	GGGACCGUGUGAGUUGCAGUGA:UCUGCAUCUCUCACGGUCCCCA	Figure 4; Table 3
CHL-I_21/21_miRNA-like	GGACCGUGUGAGUUGCAGUGA:UCUGCAUCUCUCACGGUCCCC	Figure 4; Table 3
CHL-I_22/21_miRNA-like	GGAUCCGUGUGAGUUGCAGUGA:UCUGCAUCUCUCACGG-UCCCC	Figure 4; Table 3
CHL-I_21/21	GGACCGUGAGAGAUGCAGAGC:UCUGCAUCUCUCACGGUCCCC	Figure 5
CHL-I_22/22	GGGACCGUGAGAGAUGCAGAGC:UCUGCAUCUCUCACGGUCCCCA	Figure 5, Table 5

The two strands are separated by a colon. Bold letters indicate introduced mutations or mismatches from the antisense strand sequence