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sRNAs mapping to GFP and CHL-I mRNAs after treatment of leaves of N. benthamiana with siRNAs of different structures
| Applied siRNA length (nt) (passenger/guide strands) | sRNA position relative to applied siRNA | Total sense and antisense small RNAs 19–25 nt (CPM) | |
|---|---|---|---|
| GFP | CHL-I | ||
| 21/22 | 5' | 84,701 | 1101 |
| 3' | 449,730 | 7208 | |
| 22/22 | 5' | 58,392 | 1336 |
| 3' | 294,609 | 14,175 | |
| 21/21 | 5' | 4410 | 143 |
| 3' | 3998 | 14 | |
| 22/21 | 5' | 6170 | 4450 |
| 3' | 4148 | 39 | |
| Sandpaper only | 5' | 3206 | 866 |
| 3' | 281 | 11 | |
CPM counts per million
