Table 1.
Recombinant proteins and other products produced and secreted with Priestia megaterium
| Product | Features | Product titer | Reference |
|---|---|---|---|
| Intracellular | |||
| Glucose dehydrogenase (Gdh) | Native promoter of gdh | 30 U mL−1 | (Meinhardt et al. 1989) |
| Glucose dehydrogenase (Gdh) | PxylA | 101.1 U mgProtein−1 | (Rygus and Hillen 1991) |
| Mutarotase (Mro) | 73.7 U mgProtein−1 | ||
| Urokinase-like Plas-minogen activator (Puk) | 400 U mL−1 per optical density unit | ||
| β-Galactosidase | PxylA | 4,937 mU | (Rygus and Hillen 1991) |
| PxylAopt. | 5,200 mU | (Hartz et al. 2019) | |
| Promoter of gene of putative ferrous iron transport protein | 6,300 mU | ||
| Clostridioides difficile toxin TcdA | PxylA, size: 308 kDa, his-tag | 5-10 mg L−1 | (Yang et al. 2008) |
| Clostridioides difficile toxin TcdB | PxylA, size: 270 kDa, his-tag | 10 mg L−1 | |
| Chimeric protein vaccine Tcd169 | PxylA | n.d. | (Wang et al. 2018) |
| Chimeric protein vaccine Tcd169Fl | PxylA | n.d. | |
| Chloroform reductive dehalogenase | PT7, his-tag, purification, B12-cofactor | 180 mg L−1 (calculated) | (Jugder et al. 2018) |
| Reductive dehalogenase RdhANP plus mutants | PT7, his-tag, purification, B12-cofactor | n.d. | (Payne et al. 2015) |
| Green fluorescent protein (GFP) | PxylA, Strep-tag, fed-batch, 52 gCDW L−1 | 274 mg L−1 | (Biedendieck et al. 2007c) |
| PsacB | 7.9 mg gCDW−1 | (Biedendieck et al. 2007b) | |
| PxylAopt., fed-batch, 35 gCDW L−1, ΔxylA-mutant | 1.25 g L−1 | (Stammen et al. 2010a) | |
| PT7 | 50 mg L−1 | (Gamer et al. 2009) | |
| PK1E | 61.4 mg gCDW−1 | (Stammen et al. 2010b) | |
| CbiX | PxylA, his-tag, purification | n.d. | (Leech et al. 2003) |
| CbiH60 | PxylA, his-tag | n.d. | (Moore et al. 2013a) |
| HemG | PxylA, his-tag, purification | n.d. | (Mobius et al. 2010) |
| Extracellular | |||
| Clostridioides difficile toxin TcdB | PxylA, size: 270 kDa, his-tag, SPLipA | n.d. | (Yang et al. 2008) |
| Keratinase | PxylA, native signal peptide | 186.3 U mL−1 | (Radha and Gunasekaran 2008) |
| PamyL, native signal peptide | 171.3 U mL−1 | ||
| Levansucrase SacB | PxylAopt., glycerol as C-source | 520 mg L−1 | (Korneli et al. 2013) |
| PsacB | 4252.4 U L−1 | (Biedendieck et al. 2007b) | |
| Levansucrase LevΔ773 | PxylA, SPLipA | 4 mg L−1 | (Malten et al. 2006) |
| Levansucrase LevΔ773His | PxylA, SPLipA, his-tag | 2.1 mg L−1 | |
| Levansucrase StrepLevΔ773 | PxylA, SPLipA, strep-tag | 2.7 mg L−1 | |
| Dextransucrase DsrS | PxylA, size: 188 kDa, native signal peptide | 240 U L−1 | (Malten et al. 2005b) |
| α-Cyclodextrin glycosyltransferase | PxylA, SPLipA, codon optimized | 8.9 U mL−1 | (Zhou et al. 2012) |
| Thermobifida fusca hydrolase (TFH) | PxylAopt., SPYocH | 7,200 U L−1 (7.7 mg L−1) | (Stammen et al. 2010a) |
| Endoglucanase EGI1 | PT7, 5 different signal peptides, different media | 108 mg L−1 | (Kalbarczyk et al. 2018) |
| Multimodular cellulose Cel9AT | 52 mg L−1 | ||
| Xylanase | PxylA, his+strep-tag, purification | 304.26 IU mL−1 | (Zheng et al. 2012) |
| Thermostable xylanase | PxylA, his+strep-tag, purification | 106 IU mL−1 | (Sun et al. 2015) |
| ß-glucosidase (BglZ) | PxylA | Activity measured in cell extract | (Kurniasih et al. 2014) |
| Endoglucanase (EglII) | |||
| Fusion protein EglII-BglZ | |||
| Priestia megaterium penicillin G acylase (PGA) | PxylA, SPLipA, ΔxylA-mutant | 41 mg L−1 | (Yang et al. 2006) |
| P. megaterium PGA | PxylAopt., native signal peptide, purified from growth medium, crystallization | 500 U L−1 (20.6 mgpurified enzyme L−1) | (Mayer et al. 2019) |
| Bacillus species FJAT PGA | 550 U L−1 (30.2 mgpurified enzyme L−1) | ||
| Bacillus thermotolerans PGA | 220 U L−1 (15.2 mgpurified enzyme L−1) | ||
| Hybrid PGAs | PxylAopt., native signal peptide, purified from growth medium | n.d. | (Mayer et al. 2019) |
| Single chain PGAs | n.d. | ||
| Chimeric versions of S-layer protein SslA | PxylA, cell surface display | n.d. | (Knobloch et al. 2012) |
| Antibody fragment scFV(D1.3) α-lysozyme | PxylAopt., SPLipA, his-tag, micro-bioreactor | 14 mg L−1 | (Lakowitz et al. 2017) |
| Antibody fragment D1.3 scFab α-lysozyme | PxylA, SPLipA, his-tag | 3.5 μg L−1 | (Jordan et al. 2007) |
| Whole-cell systems | |||
| Homolog Cbi-enzymes for cobalamin biosynthesis | PxylA, overexpression of 14 gene cbi-operon | Used for cobalamin production (220 μg L−1) | (Moore et al. 2014) |
| HemA | PxylA | Used for cobalamin production (2.8 μg L−1) | (Biedendieck et al. 2010) |
| HemAXCDBL | PxylA, integrated upstream of operon | Used for cobalamin production (8.5 μg L−1) | (Biedendieck et al. 2010) |
| Mannitol dehydrogenase (MDH) and formate dehydrogenase (FDH) | PxylA, 2 gene operon, codon optimized | Whole-cell transformation for D-mannitol production (22 g L−1) | (Baumchen et al. 2007) |
| Cytochrome P450 CYP106A1 | PxylAopt., coproduction with reductase Arh1 and a redox partner | Whole-cell transformation for hydroxylation of 11-keto-β-boswellic acid to 15α-hydroxy-KBA (560.7 mg L−1 day−1) | (Brill et al. 2014) |
| Cytochrome P450 CYP106A2 | PxylAopt., coproduction with redox partners AdR and Adx | Whole-cell transformation for hydroxylation of 11-keto-β-boswellic acid to 15α-hydroxy-KBA (560.7 mg L−1 day−1) | (Bleif et al. 2012) |
| Cytochrome P450 CYP109A2 | PxylAopt. | Whole-cell transformation for the conversion of vitamin D3 to 25-hydroxyvitamin D3 (54.9 mg L−1 day−1) | (Abdulmughni et al. 2017a) |
| Cytochrome P450 CYP109E1 | PxylAopt. | Whole-cell transformation for the conversion of vitamin D3 to 25-hydroxyvitamin D3 (24.5 mg L−1 day−1) | (Abdulmughni et al. 2017b) |
| Bovine cytochrome P450 CYP11A1 | PxylAopt., coproduction with redox partners AdR and Adx, codon optimized | Whole-cell transformation for the conversion of cholesterol and analogs (up to 116 mg L−1 48 h−1) | (Gerber et al. 2015) |
| Human cytochrome P450 CYP27A1 | PxylAopt., coproduction with redox partners AdR and Adx, codon optimized | Whole-cell transformation hydroxylation of cholesterol, vitamin D3 and 7-dehydrocholesterol (up to 113.14 mg L−1 48 h−1) | (Ehrhardt et al. 2016) |