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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: J Cell Physiol. 2021 Apr 14;236(10):6836–6851. doi: 10.1002/jcp.30380

FIGURE 4.

FIGURE 4.

HuR knockdown decreases TGFβ1-induced myofibroblast differentiation and ECM protein production. (A) HuR siRNA: representative western blots showing reductions in the expression of HuR, α-SMA, COL1A1, COL3A1 and FN proteins. Equal loading was confirmed with tubulin. Quantification revealed that there was reduction in α-SMA (B), COL1A1 (C), COL3A1 (D) and FN (E) protein in TGFβ1-treated HLFs transfected with HuR siRNA (SiHuR) oligos mRNA compared to control siRNA (SiCtrl)-transfected cells. Results are expressed as the mean ± SEM; n = 3 independent experiments.