Fig. 3.

General effects of FM and BM on the viability of CCD-986SK and SK-MEL-31 cells. The culture medium of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) in the flask is “FM” and that of hUC-MSCs in the bioreactor is “BM.” Cell viability was assessed with the colorimetric MTT assay. BM and FM were treated by various concentration (30%, 60%, and 100% of BM and FM) on CCD-986SK (A) and SK-MEL-31(B) to confirm cytotoxicity. The survival rate was more than 80% at each concentration and no cytotoxicity was confirmed. The control has nothing in it. Experiments were performed in triplicate.