Fig. 4.

The quantification of concentration for Hyaluronic acid, Pro-collagen, Pro-melanin in FM and BM. The culture medium of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) in the flask is “FM” and that of hUC-MSCs in the bioreactor is “BM.” The concentration Hyaluronic acid (HA), Pro-collagen, Pro-melanin was evaluated using ELISA kits. Results are expressed as respective control. Experiments were performed in triplicate. HA and Pro-collage showed high levels in BM and Pro-melanin showed high levels in FM. The experimental significance was determined by Student's t-test. Significantly differences between FM and BM were evaluated at ∗P < 0.05; and ∗∗P < 0.01.