Fig. 7.

Inhibition effects culture medium of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) extract on whitening related RNAs of melanogenesis in SK-MEL31 cells. The culture medium of human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) in the flask is “FM” and that of hUC-MSCs in the bioreactor is “BM.” Effects of FM and BM on the expression of MITF, tyrosinase, TRP-1 and TRP-2 in SK-MEL-31 cells treated with basic culture medium (Control), FM, and BM are shown. FM and BM play an important role in the expression of MIFT and tyrosinase and production of melanin. The effect on the expression of MITF, tyrosinase, TRP-1, and TRP-2 (a transcriptional promoter), was analyzed with RT-PCR. The control group is basic culture medium instead of FM and BM in SK-MEL31. Significant differences were evaluated at ∗P < 0.01.