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. 2021 Aug 26;12:5139. doi: 10.1038/s41467-021-25233-y

Fig. 3. Yeast extracts from different strain backgrounds exhibit similar metabolism and rapidly consume glucose.

Fig. 3

a Panel of cell-free reactions containing yeast extracts from 3 strain backgrounds with the optimized conditions from Fig. 2. All extracts retained metabolic activity, consuming glucose and producing ethanol and BDO. Extract from the rewired BDO Sigma strain produced the highest BDO titer and was characterized further. b Time course of cell-free reactions containing extract from the rewired BDO Sigma strain showing rapid glucose consumption. c Fed-batch reaction with additions of 45 mM glucose, 1 mM NAD, and 2 mM ATP at 1.5 h intervals. Data represent mean ± standard deviation of n = 3 technical replicates, and time courses represent separate samples quenched at each time point. Source data are provided as a Source Data file.