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. 2021 Aug 26;12(9):810. doi: 10.1038/s41419-021-04105-9

Fig. 1. Expression and subcellular distribution of MIR22HG.

Fig. 1

aMIR22HG expression in 36 pairs of cancerous and adjacent normal breast tissues detected by real-time PCR, with β-actin as internal control. b MIR22HG expression in normal breast epithelial cell line MCF10A and breast cancer cell lines MCF7, MDA-MB-231, BT549, and SKBR3 detected by real-time PCR, with β-actin as internal control. c MIR22HG expression in subcellular fractions of MDA-MB-231 cells detected by real-time PCR, with U6 and GAPDH as nuclear and cytoplasmic markers, respectively. d Fluorescence in situ hybridization analysis revealed that MIR22HG (red) was mainly located in the cytoplasm. DAPI-stained nuclei are in blue (in 63*oil lens). *P < 0.05, **P < 0.01. All experiments were repeated thrice.