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. 2021 Aug 26;12(9):810. doi: 10.1038/s41419-021-04105-9

Fig. 2. Loss-of-function of MIR22HG promotes the proliferation and migration of breast cancer cells.

Fig. 2

a Knockdown efficiency was detected by real-time PCR after transfection of MDA-MB-231 and BT549 cells with siRNAs targeting MIR22HG (MIR22HG-si1 and MIR22HG-si2) or negative control (NC) for 48 h. b, c MTT assay (5-day period) and colony formation assay (18-day period) were used to determine the viability of MDA-MB-231 and BT549 cells after MIR22HG knockdown. d Transwell assay (20-h period, 3 × 104 cells per well) was performed to evaluate the migration ability of MDA-MB-231 and BT549 cells after MIR22HG knockdown (100 × magnification). **P < 0.01, ***P < 0.001, ****P < 0.0001. All experiments were repeated thrice.