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. 2021 Aug 26;12(9):810. doi: 10.1038/s41419-021-04105-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Overexpression efficiency of MIR22HG in MDA-MB-231 and BT549 cells after transfection with MIR22HG pcDNA3.1 plasmid for 48 h. b, c MTT assay (5-day period) and colony formation assay (18-day period) were performed to determine the viability of MDA-MB-231 and BT549 cells after transfection. d Transwell assay (20-h period, 3 × 10⁴ cells per well) was used to evaluate the migration ability of MDA-MB-231 and BT549 cells after MIR22HG overexpression (100× magnification). e A subcutaneous xenograft model was utilized to explore the biological function of MIR22HG in vivo. The MDA-MB-231 cells that stably expressed MIR22HG were subcutaneously injected into 6-week-old BALB/c nu/nu female mice. After 30 days, the tumors were collected, and the tumor volume and weight were measured. **P < 0.01, ***P < 0.001, ****P < 0.0001. All experiments were repeated thrice.