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. 2021 Aug 26;11:17267. doi: 10.1038/s41598-021-96735-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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gRNA targets in the chromosome of E. coli K-12 MG1655. (a) Chromosomal map of E. coli K-12 MG1655 indicating the oriC and the cutting sites of the different CRISPR targets (CRTs). (b) Efficiency of the CRTs determined by the surviving fraction, the CRTs are ordered by gRNAs targeting non-coding vs. coding regions, non-essential vs. essential genes and the number of cutting sites. CRT0 indicated as a black bar served as a control gRNA with a non-targeting sequence.