FIG. 12.

Isoprenoid biosynthesis activities in rer2 mutants. Total lysates were prepared from RER2 (SNY9) (lanes 1 and 3), rer2-2 (SNH23-7D) (lanes 2 and 4), and Δrer2 (SMY20) (lane 5) cells, Δrer2 (SMY20) cells harboring RER2 on a single-copy plasmid (lane 6), and RER2 (SNY9) cells harboring RER2 on a multicopy plasmid (lane 7) and incubated with [1-¹⁴C]IPP and FPP at 20°C (lanes 1 and 2) or 30°C (lanes 3 to 7). The lipidic products were extracted with chloroform-methanol, spotted on a thin-layer chromatography plate, and developed with the benzene-ethyl acetate (95:5) solvent system (see Materials and Methods). Dolichol from porcine liver, ficaprenol (polyprenol from F. elastica), solanesol (all-trans-nonaprenpol), and squalene were used as standards.