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Disruption of the RER2 gene. (A) The AflII-SplI region of RER2 was replaced by the LEU2 fragment. The disrupted copy of RER2 was excised with HindIII and introduced into a diploid strain. (B) Tetrad analysis of the resulting strain (RER2/Δrer2::LEU2) at 23°C. All small colonies were Leu+, indicating that the disruption of RER2 causes a severe growth defect.
