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. 2021 Aug 26;11:17282. doi: 10.1038/s41598-021-96719-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression changes of genes involved in osteogenic differentiation after stimulation with SBF. qPCR revealed the influence of S. aureus or S. epidermidis soluble factors (SBF50, 24 h; SBF50, 72 h; SBF100, 24 h; SBF100, 72 h) on genes of osteogenic differentiation. CO served as reference (red grid line). RUNX2: (A); COL1A1: (B); ALPL: (C); SPPL: (D); BGLAP: (E). The results are presented as relative quantification compared to the CO group. The one sample t-test for a null hypothesis (H₀ = 1) was done. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.