Figure 4.

Comparison of differentiating BEL-C and BEL-P cell lines with erythroid cell differentiation from respective cord blood and adult peripheral blood CD34⁺ cells

(A) Percentage of erythroid cell types present during differentiation of BEL-C, CB, BEL-P, and PB cells during erythroid differentiation. Pre-proE, pre-proerythroblast; ProE, proerythroblast; BasoE, basophilic erythroblast; PolyE, polychromatic erythroblast; OrthoE, orthochromatic erythroblast; Retic, reticulocyte. (B) representative images from cytospins of these cultures. Scale bars, 20 μm. Arrowheads indicate the following cell types: green, Pre-proE; white, ProE; blue, BasoE; orange, PolyE; black, OrthoE; red, Retic. Data are shown as mean ± SD (n = 3). (C) flow cytometry analysis of band 3 versus α4-integrin and GPA versus CD36 cell surface expression of BEL-C, BEL-PO, CB, and PB cells during erythroid differentiation. Plots are representative of three independent cultures.