TABLE 1.

Recombination of Ty5 cDNA generated by wild-type and integrase mutant (in-611) elements in different rad strains^a

Strain	Ty5 genotype/strain genotype	His+ frequency (10−5)	His+ frequency relative to wt	Plasmid events/total (%)	Chromosomal events/total (%)
YNK366	wt/wt	8.85 ± 0.30	1.00	59/105 (56.2)	46/105 (43.8)
YNK372	wt/rad1	7.26 ± 0.12	0.82	54/145 (37.2)	91/145 (62.8)
YNK378	wt/rad52	3.34 ± 0.05	0.38	1/119 (0.8)	118/119 (99.2)
YNK384	wt/rad1 rad52	2.54 ± 0.08	0.29	1/140 (0.7)	139/140 (99.3)
YNK369	in-611/wt	1.15 ± 0.02	0.13	69/70 (98.6)	1/70 (1.4)
YNK375	in-611/rad1	1.24 ± 0.01	0.14	128/128 (100.0)	0/128 (0.0)
YNK381	in-611/rad52	<0.01	<0.01	NA	NA
YNK387	in-611/rad1 rad52	0.01 ± 0.01	<0.01	NA	NA

^aHis⁺ frequency was calculated by dividing the His⁺ cell number by the total Ura⁺ cell number. The His⁺ frequency relative to wild type (wt) was calculated by dividing the His⁺ frequencies of different strains by the His⁺ frequency of a wild-type strain carrying a wild-type Ty5. The recombination proportion was calculated by dividing the His⁺ 5-FOA^s cell number by the His⁺ cell number (column 4). The remaining His⁺ events were chromosomal events. NA, not applicable.