TABLE 2.
Effects of Ty5 PPT and U3-tip mutations on integration and recombinationa
| Strain | Ty5 genotype/strain genotypeb | His+ frequency (10−5) | His+ frequency relative to wt | Plasmid events/total (%) | Chromosomal events/total (%) |
|---|---|---|---|---|---|
| YNK366 | wt/wt | 6.23 ± 1.20 | 1.00 | 28/80 (35.0) | 52/80 (65.0) |
| YNK959 | PPT mutant/wt | 1.74 ± 0.29 | 0.28 | 76/79 (96.2) | 3/79 (3.8) |
| YNK960 | U3-tip mutant/wt | 1.76 ± 0.52 | 0.28 | 77/82 (93.9) | 5/82 (6.1) |
| YNK378 | wt/rad52 | 2.99 ± 0.88 | 0.48 | 1/89 (1.1) | 88/89 (98.9) |
| YNK961 | PPT mutant/rad52 | 0.06 ± 0.06 | 0.01 | 1/29 (3.4) | 28/29 (96.6) |
| YNK962 | U3-tip mutant/rad52 | 0.10 ± 0.05 | 0.02 | 1/26 (3.8) | 25/26 (96.2) |
a
His+ frequency and the proportion of plasmid and chromosomal events were determined as described in footnote a to Table 1.
b
U3 tip, the first two nucleotides at the 5′ end of the 3′ LTR.