TABLE 3.
Preferential recombination of Ty5 cDNA with telomeric substrates requires the domain responsible for Ty5 integration specificity
| Strain | Ty5 location | No. of chromosomal recombination events/no. of plasmid events (frequency)a
|
||
|---|---|---|---|---|
| Ty5 in-611
|
Ty5 in-611,1094
|
|||
| TP assay | TF with Ty5-HIS3 | TP assay | ||
| W303-1A | No Ty5 | 1/22 (0.05) | 2/33 (0.06) | 0/24 (0.00) |
| YNK570 | Internal; W3-AI | 11/18 [4/10] (0.24) | 41/31 (1.32) | 13/12 [4/10] (0.43) |
| YNK567 | Telomere; W2-AI | 27/14 [7/10] (1.35) | 14/19 (0.74) | 25/29 [8/9] (0.77) |
| YNK568 | Telomere; W77-AI | 45/24 [9/10] (1.69) | 19/32 (0.59) | 18/39 [9/10] (0.42) |
| YNK569 | Telomere; W84-AI | 26/23 [8/10] (0.90) | 28/14 (2.00) | 21/37 [9/10] (0.51) |
a
TP, transposition; TF, transformation; in-611, DD(35)E mutant of Ty5 integrase; in-611,1094, DD(35)E and targeting domain double mutant; Ty5-HIS3, Ty5 element with the functional HIS3 gene. The number in brackets is the proportion of chromosomal recombination based on PCR analysis; this proportion was multiplied by the ratio of chromosomal events to plasmid events to give the frequency.