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. 2021 Aug 26;12:5123. doi: 10.1038/s41467-021-25107-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Affinity purification mass spectrometry (AP-MS) strategies for identification of OCT4-associated proteins (interactome) in hESCs. Three strategies are used: (i) streptavidin immunoprecipitation (SA-IP) of WIBR2-OCT4^3xFLBio ESCs or BirA control; (ii) FLAG-IP of WIBR2-OCT4^3xFLBio ESCs or BirA control; (iii) native OCT4 and IgG antibody (Ab-IP) pulldown in WIBR2 and WIBR3 hESC lines. b OCT4-associated proteins identified in naïve and primed hESCs. Proteins are listed as official gene symbols. c Proteins in the naïve human OCT4 interactome are compared to OCT4-associated proteins identified in three independent studies in mouse ESCs^29–31. Components of SNF2-family chromatin remodeling complexes are underlined. d OCT4 co-immunoprecipitation (co-IP) followed by western blot of selected OCT4-associated proteins in naïve and primed hESCs. e BRM (SMARCA2) and BRG1 (SMARCA4) co-IP followed by western blot analysis of BAF155 (SMARCC1) and OCT4. d, e Experiment is repeated independently twice with similar results.