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. 2021 Aug 26;12:5123. doi: 10.1038/s41467-021-25107-3

Fig. 2. Differential expression of OCT4-associated SNF2-family ATPases in naïve and primed hESCs.

Fig. 2

a Illustration of naïve-specific (red/pink), primed-specific (blue), and common (yellow) components of the mammalian SWI/SNF family of subunits (represented as one complex rather than three subcomplexes cBAF, PBAF, and ncBAF) and ISWI complexes identified in the naïve and primed OCT4 interactomes. Pink color indicates proteins that are identified with marginal significance (0.1 < FDR < 0.2) in the naïve OCT4 interactome. b Western blot analysis of ATPase expression in naïve and primed hESCs. Experiment is repeated independently twice with similar results. c log2 ratios of mRNA expression of BAF and ISWI components in naïve and primed hESCs based on a microarray analysis4. d Expression of BRG1 (SMARCA4), BRM (SMARCA2), and SNF2L (SMARCA1) mRNA transcripts in the alternative t2iLGoY culture condition for naïve human pluripotency (red) and parental primed hESCs (blue) according to RNA-seq data5,46. e log2 ratios of protein expression of BAF and ISWI components in naïve and primed hESCs based on SILAC followed by MS analysis in two naïve and primed hESC lines. *SNF2L was quantified by MS only in WIBR2 cells.