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. 2021 Aug 26;12:5126. doi: 10.1038/s41467-021-25186-2

Fig. 5. ISL1 in amnion-like cells regulates human mesodermal cell formation through BMP4.

Fig. 5

See also Supplementary Fig. 9-12. a Diagram of the transwell assay. b immunofluorescent staining for ISL1 in AMLCs. Scale bar 50 µm. n = 4 biologically independent experiments c expression of BMP4 in AMLCs. Data are presented as mean ± SEM and analyzed by two-tailed Student’s t test. n = 6 biologically independent samples. *p value <0.05 (<0.0001 for wild type versus ISL1-null and 0.0002 for ISL1-null versus ISL1-null + mod-ISL1). d immunofluorescent staining for the mesoderm marker brachyury (BRA, green) in MeLCs. Nuclei stained with DAPI (blue). Scale bar 100 µm. e Quantification of BRA+ cells from (d). Data are presented as mean ± SEM and analyzed by two-tailed Student’s t test. n = 11 biologically independent samples. *p value <0.05 (<0.0001 for wild type versus ISL1-null and 0.0002 for ISL1-null versus ISL1-null + mod-ISL1). f immunofluorescent staining for BRA in wild-type MeLCs without or with Noggin treatment. Scale bar 100 µm. n = 3 biologically independent experiments. g Immunofluorescent staining for BRA in ISL1-null MeLCs without or with BMP4 treatment. Scale bar 100 µm. n = 6 biologically independent experiments. AMLCs amniotic ectoderm-like cells, MeLCs mesoderm-like cells.