Subject	Biomaterials
Specific subject area	Silk Fibroin Fibers properties and their correlation with the degumming process.
Type of data	Table
How data were acquired	Analytic Balance, Kern ABS. Perkin Elmer Nano Drop UV spectrophotometer. Fourier-transform infrared - Attenuated Total Reflectance spectroscopy (FTIR-ATR), Perkin Elmer, Spectrum ONE equipped with a ZnSe crystal. Universal testing machine, Nanotensile machine Agilent T150. Gel permeation chromatography system (GPC): SB-806MHQ Shodex column (8.0 × 300 mm Exclusion limit [Pullulan] 4000E3) mounted on the pumping system (spectra system p4000 with a 20 µL loop and a vacuum membrane degasser thermo Electron SCM1000), UV detecting system Jasco UV-1570.
Data format	Dataset of the extrapolated data from the raw curve and dataset including the raw curves.
Parameters for data collection	The FTIR spectra were collected on each sample by mediating 16 scans in the 400–4000 cm−1 range with a resolution of 1 cm−1. The mechanical test of the fiber was done in controlled conditions of 20–21 °C and relative humidity 39–42%. The gel permeation chromatograms were collected by keeping the eluent at 27 °C throught a custom-built oven.
Description of data collection	The Weight loss (%) was obtained by weight difference. The Sericin removed (%) was obtained by UV-spectrophotometry. Ultimate Strength (MPa), Ultimate Strain (%), Young's modulus (MPa), Toughness modulus (MJ/m3) were obtained by analyzing the stress-strain curves. The fibers diameter were obtained by optical microscopy. The Relative amount of secondary structures (%) were obtained by FTIR-ATR. The Peak Molecular Weight (kDa), the Number average Molecular Weight (kDa), and Weight average Molecular Weight (kDa) were obtained by analyzing the chromatographs obtained by GPC.
Data source location	Institution: BIOTech Research Center, University of Trento City/Town/Region: Trento Country: Italy Latitude and longitude (and GPS coordinates, if possible) for collected samples/data: Latitude 46.0748° N, Longitude 11.1217° E
Data accessibility	Data is available in a public repository. Repository name: IEEE Dataport Data identification number: 10.21227/emar-9460 Direct URL to data: https://ieee-dataport.org/open-access/full-factorial-design-experiment-dataset-silk-fibroin-alkaly-degumming Instructions for accessing these data: Data is freely and openly available at the reported link. The use of the data is regulated by the creative commons.
Related research article	Alessio Bucciarelli, Gabriele Greco, Ilaria Corridori, Nicola M. Pugno, and Antonella Motta, A Design of Experiment Rational Optimization of the Degumming Process and Its Impact on the Silk Fibroin Properties, ACS Biomater. Sci. Eng. 2021, 7, 4, 1374–1393. 10.1021/acsbiomaterials.0c01657