Table 3.
The LT50 determination and summary of probit analysis to determine LC50 and LC90 concentrations of Beauveria bassiana strains against the third instar of Tuta absoluta larvae in 10 days post incubation
LT50 (mean ± SE) | ||||||||
---|---|---|---|---|---|---|---|---|
AAUB03 | AAUB29 | AAUB28 | AAUB76 | AAUB59 | AAUB39 | AAUB19 | AAUB90 | |
Spores·ml-1 | ||||||||
104 | 3.33± 0.58a | 5.33 ± 0.58c | 3.33± 0.58a | 5.67 ± 0.58cd | 5.00 ± 1.00c | 3.67 ± 0.58ab | 3.33 ± 0.58a | 5.00 ± 1.00c |
106 | 2.33± 0.58a | 2.57 ± 0.51a | 2.50± 0.00a | 2.83 ± 0.29ab | 2.93 ± 0.12ab | 2.17 ± 0.29a | 2.33 ± 0.29a | 4.00 ± 0.00c |
108 | 1.93± 0.12a | 2.00 ± 0.00a | 2.20± 0.35a | 2.33 ± 0.29a | 2.50 ± 0.50ab | 2.00 ± 0.35a | 2.00 ± 0.62a | 3.17 ± 0.29c |
Summary of probit analysis | ||||||||
LC50 | 5.3 × 103 | 6.5 × 104 | 1.5 × 103 | 9.3 × 104 | 1.1 × 105 | 1.8 × 104 | 1.8 × 103 | 2.6 × 103 |
95% FL | 1.3 × 103 –7 × 104 | 2.2 × 104–1.5 × 105 | 8.6 × 102–4.9 × 103 | 9.9 × 103–1.3 × 104 | 2 × 104–3.3 × 105 | 3.9 × 103–6 × 104 | 1 × 103–6.1 × 104 | 1.2 × 103–3.1 × 104 |
LC90 | 2.8 × 105 | 1.7 × 106 | 2.9 × 105 | 1.6 × 106 | 1.8 × 107 | 1.6 × 107 | 7.6 × 105 | 1.2 × 107 |
95% FL | 2.1 × 105 –6.3 × 106 | 6.3 × 105–1.1 × 107 | 1.9 × 104–2.9 × 106 | 1.8 × 105–2.7 × 107 | 4.2 × 106–2.8 × 108 | 2.2 × 106–3.1 × 108 | 7.1 × 104–2.1 × 107 | 1.5 × 106–7.3 × 108 |
Int ± SE | 1.32 ± 0.91 | 4.33 ± 0.89 | 0.85 ± 0.83 | 1.16 ± 0.72 | 2.91 ± 0.66 | 1.54 ± 0.64 | 0.8 ± 0.75 | 1.19 ± 0.63 |
S ± SE | 0.49 ± 0.18 | 0.9 ± 0.17 | 0.39 ± 0.16 | 0.39 ± 0.13 | 0.58 ± 0.11 | 0.39 ± 0.11 | 0.36 ± 0.14 | 0.35 ± 0.11 |
p value | < 0.001 | < 0.001 | < 0.012 | < 0.003 | < 0.001 | < 0.001 | < 0.001 | < 0.001 |
Legends: FL, fiducial limit; SE, standard error; LT, lethal time taken to kill fifty of experimental organisms; LC, lethal concentration; Int, intercept; S, slope. The same letters in the columns (a, b, c, d, e, and f) showed the mean values without significant difference at p ≤ 0.05