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. 2021 Aug 26;11:17205. doi: 10.1038/s41598-021-96298-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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K_m and IC₅₀ determinations for YePEPT^K314A-F311Y. (a) Kinetics of [³H]Ala-Ala uptake in E. coli cells transformed with YePEPT^K314A-F311Y. IC₅₀ determination of YePEPT^K314A-F311Y for (b) amoxicillin, (c) cefadroxil and (d) cefprozil by heterologous competition. Data points in (a) represent vector-subtracted uptake of [³H]Ala-Ala normalized to V_max ± SEM of three experiments, each at least in triplicate. Data points in (b)–(d) represent vector-subtracted uptake of [³H]Ala-Ala normalized to the uninhibited signal ± SEM of three independent experiments, each at least in triplicate. Numbers in brackets below K_m- and IC₅₀-values represent 95% confidence intervals in µM.