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Analysis of endogenous FGF-2 isoforms in HeLa and SK-Hep-1 cells. Aliquots (20 μg) of cell extracts from HeLa and SK-Hep-1 cells were analyzed by PAGE and transferred to nitrocellulose (see Materials and Methods). Immunoblotting was performed with anti-FGF-2 antibodies and chemiluminescence detection, immediately followed by autoradiography for 2 h. Positions of migration of size standards (right) and of the FGF-2 isoforms (left) are indicated.
