FIG. 2.

Identification of the 34-kDa FGF-2 with anti-N-terminal antibodies. (A) Schema representing the FGF-2 mRNA and the FGF-2 coding sequence, showing positions of the peptides used to prepare the anti-N23 and anti-FGF-2 polyclonal antibodies (see Materials and Methods). (B) Heparin-Sepharose-purified HeLa extract was analyzed by Western immunoblotting with either anti-N23 (αN23) or anti-FGF-2 (αFGF-2) antibodies (see Materials and Methods). Migration of size standards is indicated on the left; migration of FGF-2 isoforms is indicated on the right. The 16-kDa-migrating band revealed in lane 1 by anti-N23 probably corresponds to a cleavage product.