FIG. 7.

Expression of the 34-kDa FGF-2 from bicistronic vectors. Bicistronic vectors expressing CAT as first cistron (ORF1) and FGF-2 as second cistron (ORF2) were used to analyze the cap dependence of the 34-kDa FGF-2. (A) Representation of the two constructs with or without the presence of a 5′ hairpin. (B) Transfection of COS-7 cells with the bicistronic constructs. CAT expression was analyzed by measuring CAT activity (lanes 1 and 2). FGF-2 expression was analyzed by Western immunoblotting with anti-FGF-2 antibodies (lanes 3 and 4). The presence or absence of a hairpin is indicated by + or −, respectively. Migration of the different FGF-2 isoforms is shown on the right.