Table 1.

Summary of constructs used

ORF1 construct	Domain organization	Phase separation in comparison with wild-type ORF11–338	Retrotransposition	κ-Value (41)
ORF11–338	full length	comparable	normal	0.149
ORF11–53	N-terminal region	none	not determined	–
ORF11–152	N-terminus + coiled coil	comparable	not determined	–
ORF153–152	coiled coil	none	not determined	–
ORF153–338	ΔN-terminus	reduced	none (19)	–
ORF166–338	ΔN-terminus + truncated coiled coil	reduced	none (19)	–
ORF1157–338	RRM + CTD	none	not determined	–
ORF1–338 K3A/K4A	full length	comparable	not determined	K3A = 0.147
			ORF1–338 G2A/K3A/K4A: none (42)	K4A = 0.146
			ORF11–338 K3R: increased (19)	K3A/K4A = 0.146
ORF1–338 K3E/K4E	full length	reduced	not determined	K3E = 0.144
				K4E = 0.142
				K3E/K4E = 0.144
ORF1–338 K3A/K4A/R7A/K8A	full length	reduced	not determined	0.143
ORF1–338 K3E/K4E/R7E/K8E	full length	reduced	not determined	0.151
ORF1–338 R7E/K8E	full length	reduced	not determined	0.143
ORF1–338 S27D	full length	increased	increased (43)	0.150
ORF1–338 L93P	full length	increased	not determined	0.149
			ORF1–338 L93N/L199N: none (19)
			ORF11–338 E89A/L90A/M91A: none (42)
			ORF11–338 E92A/L93A/K94A: none (42)