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. 2021 Apr 20;120(11):2222–2236. doi: 10.1016/j.bpj.2021.04.007

Figure 2.

Figure 2

Examination of steady-state FRET as a function of nucleotide state. Representative wavelength scans used to perform FRET efficiency measurements of M2β-S1 A488 RLC with Cy3ATP and Cy3ADP. (A) The fluorescence spectrum of donor-acceptor, donor only, and acceptor only is shown. (B) The fluorescence spectrum of donor only compared with donor-acceptor and comparing Cy3ATP and Cy3ADP. The FRET efficiency was calculated after correcting for the fraction of Cy3 nucleotide bound, using the KD estimated from stopped-flow measurements (Fig. 1C). A summary of the FRET efficiency measurements and associated distances is shown in Table 1.