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. 2021 Apr 20;120(11):2222–2236. doi: 10.1016/j.bpj.2021.04.007

Figure 7.

Figure 7

Impact of R723G and F764L mutants on lever arm conformation monitored by FRET. Measurements of Cy3ATP and Cy3ADP binding to M2β-S1 were examined as described in Fig. 1. The amplitude of the FRET change was plotted as a function of Cy3ATP or Cy3ADP concentration and fitted to a hyperbolic function for (A) R723G and (B) F764L. (C) Summary of steady-state FRET efficiency determined from measuring the emission spectra of 0.5 μM M2β-S1 in the presence of 1 μM Cy3ATP or Cy3ADP in WT, R723G, and F764L (average of six repeats from three protein preparations). The black bar represents the mean ± SD. Unpaired Student’s t-tests were performed to compare each mutant with WT, and no significant differences were found between the mutants and WT.