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. 2021 Apr 20;120(11):2222–2236. doi: 10.1016/j.bpj.2021.04.007

Figure 9.

Figure 9

Loaded in vitro motility of M2β-S1 WT, R723G, and F764L. The impact of a tethering load imposed by the presence of M2β-S1 E466A (motor dead) was examined in WT, R723G, and F764L M2β-S1 from three different protein preparations. The total concentration of M2β-S1 loaded onto the motility surface was kept constant at 0.6 μM. Velocities were analyzed by the program FAST (44). (A) The sliding velocity was expressed relative to 0% motor dead and plotted as a function of the fraction of motor dead present. (B) The sliding velocity was plotted as a function of the tethering load produced by the presence of motor-dead myosin and converted to force (piconewtons) as described in the Materials and methods. (N = 6, error bars represent SEM). Unpaired Student’s t-tests were performed to compare each mutant with WT at each [motor dead]% (∗p < 0.05).