Figure 2.

Prolonged shear stress induces differential actin-cytoskeleton rearrangements upstream and downstream of flow. (A) Representative z-projection confocal microscopy images (top) and magnified images (bottom) of the actin cytoskeleton on TNFα-stimulated ECs in the absence (left) and presence of 4 h continuous shear flow (right). Vertical white arrows on the right indicate the flow direction. Scale bars, 20 μm. (B) Representative STORM images (top) and corresponding magnified images (bottom) of the actin cytoskeleton in static TNFα-treated ECs (left) and after 4 h of shear-flow exposure (right). Vertical white arrows indicate the direction of the flow. Small white arrows point to actin patch-like structures. Scale bars, 5 μm (full images) and 1 μm (zoom regions). To see this figure in color, go online.