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. 2021 Jun 2;120(13):2665–2678. doi: 10.1016/j.bpj.2021.05.021

Figure 1.

Figure 1

Live-cell imaging of MuSCs within their niche at slack length and high-strain myofiber bundles. (A, B, I, and J) Micrographs of typical MuSCs in their native niche within myofiber bundle at slack length and at high strain (~40% above slack length) from EDL and SO muscles. (C, D, K, and L) Measurement of MuSC length change in the XY plane. White lines: length of MuSCs at myofiber bundle slack length. Red arrows: increase in length at extended myofiber bundle length. (E–H and M–P) 3D reconstructions of MuSCs at slack length and high myofiber strain illustrating MuSC shear deformations in the XY and YZ planes. Stretching of the myofiber bundles caused displacement of cell regions (red circles) in the direction indicated by the arrows (red) in the XY plane, implicating shearing of MuSC (E and F). (G, H, O, and P) Differences in MuSC length changes at both lateral sides indicate an increase in shear angle (i.e., angle of the dashed lines in YZ plane). Scale bars, 20 μm. To see this figure in color, go online.