Figure 1.

Techniques used to probe nuclei. (a) AFM indentation: cantilever with a bead attached at the tip for probing the nuclei is shown. Scale bars, 10 μm. (b) OT setup: a nucleus is either moved toward the trapped bead longitudinally (from the top) or laterally (from the side), producing an indentation on the nuclei. The schematic of the experimental procedure shows the bead deflection. Scale bars, 5 μm. (c) Microaspiration experiments and analysis are shown. (Left image) Schematic of the deformation parameters used for the solid elastic model is shown (see text for definitions). (Right image) Example of an experimental current trace showing two aspiration events, and (bottom right) the corresponding video frames for the first event where frame A is the first frame of aspiration, frame B is the last frame of aspiration, and frame C is when no aspiration is occurring. Scale bars, 5 μm. To see this figure in color, go online.