Figure 2.

Level of HP1α protein in MCF7 cells with constitutive HP1α KD. (a) Immunoblot showing HP1α expression in MCF7 cells that constitutively express either an HP1α shRNA or the matched scrambled shRNA control. Increasing amounts of total cell lysates, (1×) 25 μg and (2×) 50 μg, are loaded. The immunoblot was stained with antibodies against HP1α and α-tubulin as a loading control. (b) Immunofluorescence confocal microscopy images of MCF7 control (top panel) and MCF7 HP1α KD (bottom panel) cells stained with DAPI to detect DNA (cyan) and an antibody directed against HP1α (magenta) are shown. Scale bars, 20 μm. To see this figure in color, go online.