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. 2021 Jun 2;120(13):2631–2643. doi: 10.1016/j.bpj.2021.05.017

Figure 4.

Figure 4

Knockdown of HP1α alters the lamin A/C network. (a) Representative confocal microscopy images of z-stack maximal intensity projections of MCF7 control and MCF7 HP1α KD cells probed with an antibody against Lamin A/C are shown. Scale bars, 20 μm. (b) Representative confocal microscopy medial sections of individual nuclei from MCF7 control and MCF7 HP1α KD cells stained with antibodies against Lamin A/C and H3K9me2 are shown. Top panel: medial slice showing Lamin A/C (magenta) alone. Bottom panel: Lamin A/C (magenta) overlaid with H3K9me2 (yellow) is shown. Above each medial slice are line plot profiles of fluorescent intensity (percentage of gray value saturation). Scale bars, 5 μm. Additional images of cells are presented in Figs. S6 and S7. (c) Whole-cell lysates from an equal number of MCF7 control or HP1α KD cells were analyzed by immunoblotting with an antibody against Lamin A/C phosphorylated on serine 22 (top panel) and Lamin A/C (bottom panel). To see this figure in color, go online.