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(a) Representative scanning electron micrographs of nuclei isolated from MCF7 control and MCF7 HP1α KD cells are shown. Scale bars, (top panel) 30 μm and (bottom panel) 5 μm. (b) AFM indentation: three sweeps were applied between zero and maximal forces of 300 pN (black circle), 500 pN (green circle), and 1000 pN (blue circle) on MCF7 control and HP1α KD nuclei. The Hertzian fit to the curves is shown in Fig. S10. To see this figure in color, go online.
