Figure 7.

OT Indentation experiments. (a) Stage and bead displacements: $SD$ is represented by the blue curve. MCF7 control and HP1α KD nuclei $BD$ are represented by red and green curves, respectively. Trap stiffness is calculated from interval A, in which the bead is freely diffusing in the optical trap. The indentation D on the nuclei is extracted from interval B. (b) This figure represents the measured $BD$ (green), calculated F (red), and D (black) during the indentation interval, when the bead interacts with MCF7 control and HP1αKD nuclei, respectively. (c) $F-D$ curves extracted from the indentation interval for longitudinal indentation (from the top). (d) $F-D$ curves extracted from the indentation interval for lateral Indentation (from the side). To see this figure in color, go online.