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. 2020 Nov 1;43(4):331–340. doi: 10.1016/j.pld.2020.10.002

Fig. 3.

Fig. 3

WRKY75 directly regulates the expression of SAG12 and SAG29. (A) The promoter structure of the SAG12 and SAG29 genes and fragment used in the ChIP assay. (B) WRKY75 directly binds to the promoters of SAG12 and SAG29. ChIP assays were performed with chromatin prepared from WRKY75:YFP-WRKY75:3′-WRKY75 transgenic plants, using an anti-GFP antibody (IP). ChIP results are presented as a percentage of input DNA. Values are mean ± SD of three independent biological replicates. Asterisks indicate Student's t-test significant differences as compared to controls, ∗∗P < 0.01. (C) Schematic of the SAG12:NLS-GFP and SAG29:NLS-GFP reporters and WRKY75 and GUS effectors. (D) Transient expression assays showed that WRKY75 activates the expression of SAG12 and SAG29 determined by qRT-PCR analysis. Values are means ± SD of three independent biological replicates. ∗∗P < 0.01, Student's t-test compared with controls. These experiments were performed three times with similar results.