Fig. 4.

GAI and RGL1 repress WRKY75 activation ability. (A) Schematic of the SAG12:NLS-GFP reporter and WRKY75, RGL1, GAI and GUS effectors. (B) Transient expression assays showed that RGL1 and GAI repress transcriptional activation of WRKY75 determined by qRT-PCR analysis. Values are mean ± SD of three independent biological replicates. Asterisks indicate Student's t-test significant differences as compared to controls, ∗∗P < 0.01. (C) RGL1 interferes the binding of WRKY75 to its target genes (shown in Fig. 4A). ChIP assays were performed with chromatin prepared from Myc-WRKY75/RGL1 plants, using an anti-Myc antibody (IP). ChIP results are presented as a percentage of input DNA. Values are mean ± SD of three independent biological replicates. Asterisks indicate Student's t-test significant differences as compared to controls, ∗∗P < 0.01. These experiments were performed three times with similar results.