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. 2020 Nov 1;43(4):331–340. doi: 10.1016/j.pld.2020.10.002

Fig. 7.

Fig. 7

WRKY75 positively regulates WRKY45 expression during leaf senescence. (A) qRT-PCR analysis of transcript levels of WRKY45 in the indicated genotypes. Values are means ± SD of three independent biological replicates. ∗P < 0.05, ∗∗P < 0.01, Student's t-test compared with Col-0. (B) The promoter structure of WRKY45 gene and fragment used in the ChIP assay. (C) WRKY75 directly binds to the promoter of WRKY45. ChIP assays were performed with chromatin prepared from WRKY75:YFP-WRKY75:3′-WRKY75 transgenic plants, using an anti-GFP antibody (IP). ChIP results are presented as a percentage of input DNA. Values are mean ± SD of three independent biological replicates. Asterisks indicate Student's t-test significant differences as compared to controls, ∗∗P < 0.01. These experiments were performed three times with similar results.