Figure 2.

405-nm stimulation triggers two distinct calcium signaling responses in class IV neurons. (A and B) When the laser is focused adjacent to but not on a dendrite (noncontact), large calcium changes are recorded in the axon (green ROI), and only very small changes are recorded in the dendrite (blue ROI). (C and D) When the laser is focused on a dendrite (contact), large calcium changes are recorded in both the axon (green ROI) and dendrite (blue ROI). (E) Magnitude of normalized fluorescence responses across all seven ROIs (open circles color coded as in Fig. 1E) for a cell stimulated three consecutive times at different locations. The first two stimulations did not make contact with the dendritic arbor and evoked axonal responses only (green); the third stimulation made contact and evoked responses everywhere. Black lines indicate means for all ROIs combined. N represents the number of larvae; n represents the number of cells. To see this figure in color, go online.