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Puncta density per image area
| Ligand Treatment | Number of Puncta per Image Area (Average ± SD) | Puncta Area per Image Area (Average ± SD) |
|---|---|---|
| S1 | (2.4 ± 0.2) × 10−3 | (0.120 ± 0.009) × pixel−1 |
| S2 | (2.4 ± 0.3) × 10−3 | (0.120 ± 0.010) × pixel−1 |
| S3 | (3.0 ± 0.2) × 10−3 | (0.150 ± 0.015) × pixel−1 |
| S1 versus S2 t-statistic (confidence level) | 1.94 (5.45%) | 0.18 (85.8%) |
| S1 versus S3 t-statistic (confidence level) | 17.12 (<0.01%) | 14.27 (<0.01%) |
S1, no treatment; S2, 10-min treatment; S3, 30-min treatment.
