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. 2021 Jun 30;120(15):2984–2997. doi: 10.1016/j.bpj.2021.06.023

Figure 5.

Figure 5

Formin chimeras with poor nucleation efficiency exhibit delays in ring assembly. (A) Schematic of fission yeast cytokinesis: 1) node assembly, 2) ring assembly, 3) ring maturation, 4) ring constriction, and 5) cell separation. (B) Representative time-lapse micrographs of the morphology (DIC) and contractile ring (Rlc1-tdTomato) of representative formin chimera cells. Time is indicated in minutes. Spindle pole bodies are labeled with Sad1-tdTomato. Blue arrows indicate time of SPB separation. Yellow boxes indicate broad band of precursor cytokinesis nodes. Scale bar, 5 μm. (C) Time required for at least 10 cells to complete the entire time course of cytokinesis for each strain. (D–G) Time required for at least 10 cells to complete the indicated step of cytokinesis for each strain. (D) Frequency of cells that assemble a sharp ring over time. (E) Frequency of cells that complete ring maturation. (F) Frequency of cells that complete ring constriction. (G) Frequency of cells that complete cell separation. The p-values for pairwise log-rank tests associated with plots in (C–G) are listed in Table S4.